ParoReal® Kit Trichinella spiralis & britovi

ParoReal® Kit Trichinella spiralis & britovi

Product description

Kit contents:

• Multiplex detection assay for Trichinella spiralis & T. britovi
• Detection assay for internal positive control (control of amplification)
• DNA reaction mix (contains uracil-N glycosylase, UNG)
• Positive control for Trichinella spiralis & T. britovi
• Water

Background: Trichinellosis is a food-borne parasitic zoonosis caused by nematodes of the genus Trichinella. Twelve genotypes of Trichinella have been identified worldwide four of which are confirmed to exist in Europe: T. spiralis, T. nativa, T. britovi and T. pseudospiralis. Two main clades are recognized in the genus: one group (T. britovi, T. murrelli, T. nativa, T. nelsoni, T. spiralis) that encapsulates in host muscle tissue and a second (T. papuae, T. pseudospiralis, T. zimbabwensisi) that does not.

Description: ParoReal Kit Trichinella spiralis & britovi is based on the amplification and detection of the internal transcribed spacer of Trichinella spiralis and T. britovi using real-time PCR. Trichinella spiralis is detected in FAM channel and Trichinella britovi is detected in VIC/HEX channel.

PCR-platforms: ParoReal Kit Trichinella spiralis & britovi is developed and validated for the ABI PRISM^® 7500 instrument (Life Technologies), LightCycler^® 480 (Roche) and Mx3005P^® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Sensitivity and specificity: See product description