ParoReal® Kit Echinococcus multilocularis

ParoReal® Kit Echinococcus multilocularis

Product description

Kit contents:

• Detection assay for Echinococcus multilocularis
• Detection assay for internal positive control (control of amplification)
• DNA reaction mix (contains uracil-N glycosylase, UNG)
• Positive control for E. multilocularis
• Water

Background: Echinococcus multilocularis is a cyclophyllid tapeworm causing echinococcosis, a disease that affects many canid species including wolves, coyotes, foxes, jackals and domesticated dogs. Humans can also contract this disease. Echinococcus multilocularis requires two types of hosts to mature properly. It is transmitted to intermediate hosts via the ingestion of eggs in infected food. The eggs travel to the lungs, liver, and other internal organs where they develop into multilocular cysts. Finally, Echinococcus multilocularis is transmitted to definitive hosts by means of eating infected, cyst-containing organs. There it becomes attached to the tissues and grows.

Description: ParoReal Kit Echinococcus multilocularis is based on the amplification and detection of the mitochondrial ND1 gene of E. multilocularis using real-time PCR.

PCR-platforms: ParoReal Kit Echinococcus multilocularis is developed and validated for the ABI PRISM^® 7500 instrument (Life Technologies), LightCycler^® 480 (Roche) and Mx3005P^® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Sensitivity and specificity: See product description