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DKW/Juglans medium
Product code : DUC.D0246.0005 DUC.D0246.0005
DKW medium has been defined for in vitro propagation of Paradox Walnut Rootstock (Juglans hindsii x J. regia) via nodal explants. The explants were placed on medium without growth-regulators for one week and subsequentely on medium containing…
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DKW/Juglans medium
Product description
DKW medium has been defined for in vitro propagation of Paradox Walnut Rootstock (Juglans hindsii x J. regia) via nodal explants. The explants were placed on medium without growth-regulators for one week and subsequentely on medium containing 6-BAP and IBA. Opti-mum shoot development was supported under 4.5 µM 6-BAP and 5 nM IBA. The basal ends of the tissue culture derived shoots were dipped in 5 mM IBA solution and subsequen-tly rooted within 10 to 14 days in the greenhouse.
Composition
| Micro Elements | mg/l | µM |
|---|---|---|
| CuSO4.5H2O | 0.25 | 1.00 |
| FeNaEDTA | 44.63 | 121.61 |
| H3BO3 | 4.80 | 77.63 |
| MnSO4.H2O | 33.80 | 200.00 |
| Na2MoO4.2H2O | 0.39 | 1.61 |
| ZnSO4.7H2O | 17.00 | 72.19 |
| Macro Elements | mg/l | mM |
|---|---|---|
| CaCl2 | 112.50 | 1.01 |
| Ca(NO3)2.2H2O | 1664.64 | 8.30 |
| KH2PO4 | 265.00 | 1.95 |
| K2SO4 | 1559.00 | 8.95 |
| MgSO4 | 361.49 | 3.00 |
| NH4NO3 | 1416.00 | 17.70 |
Total concentration Micro and Macro elements: 5479.50 mg/l
river, J.A., Kuniyuki, A.H. In Vitro Propagation of Paradox walnut Rootstock, Hort. Science, 19(4), August 1984.
DKW/Juglans medium
Product specifications
Packaging Unit
5l
Brand
Duchefa
DKW/Juglans medium
Product documents