BactoReal® Kit Brachyspira hyo/pilo

BactoReal® Kit Brachyspira hyo/pilo

Product description

Kit contents:

• Detection assay for B. hyodysenteriae and B. pilosicoli
• Detection assay for internal positive control (control of amplification)
• DNA reaction mix (contains uracil-N glycosylase, UNG)
• Positive controls for B. hyodysenteriae and B. pilosicoli
• Water

Background: Brachyspira are anaerobic intestinal spirochaetes. Brachyspira hyodysenteriae (previously called Serpulina hyodysenteriae or Treponema hyodysenteriae) is the causative agent of swine dysentery. Swine dysentery is a disease characterized by mucohaemorrhagic diarrhoea with lesions confined to the large intestine of pigs. Its incubation time is 10-14 days. The faecal excretion of B. hyodysenteriae starts 2 days after infection. Brachyspira pilosicoli (previously called Serpulina pilosicoli) colonizes the large intestine of various species, including humans. It is the etiologic agent of human and animal intestinal spirochetosis and is rarely implicated as a cause of bacteremia. For example, it is the causative agent of porcine colonic spirochaetosis (PCS).

Description: BactoReal^® Kit Brachyspira hyo/pilo is based on the amplification and detection of the nox gene of B. hyodysenteriae and B. pilosicoli using real-time PCR. Other Brachyspira species are not detected by this test.

PCR-platforms: BactoReal^® Kit Brachyspira hyo/pilo is developed and validated for the ABI PRISM^® 7500 instrument (Life Technologies), LightCycler^® 480 (Roche) and Mx3005P^® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Sensitivity and specificity: See product description