BactoReal® Kit Bartonella spp.

BactoReal® Kit Bartonella spp.

Product description

Kit contents:

• Detection assay for Bartonella spp.
• Detection assay for internal positive control (control of amplification)
• DNA reaction mix (contains uracil-N glycosylase, UNG)
• Positive control for Bartonella henselae
• Water

Background: Bartonella species are gram-negative, fastidious bacteria. In addition to dogs and cats, numerous domestic and wild animals, including bovine, canine, and rodent species as well as humans can serve as a reservoir host for various Bartonella species. Cats may harbor B. henselae, B. clarridgeiae and rarely other Bartonella species. Dogs may carry Bartonella vinsonii subsp. berkhoffii, Bartonella henselae and others. Humans can be a reservoir or an incidental host for B. bacilliformis, B. clarridgeiae, B. elizabethae, B. grahamii, B. henselae, B. koehlerae, B. quintana, B. rochalimae, B. vinsonii and B. washoensis. Bartonella bacilliformis causes Carrion's disease (Oroya fever and Verruga peruana). Bartonella henselae, which frequently causes chronic bacteremia in cats, is the most common etiologic agent of cat scratch disease. It can also cause bacillary angiomatosis, peliosis hepatitis, and sometimes endocarditis in immunocompromised human patients. Bartonella clarridgeiae can cause chronic bacteremia in cats and has been associated with cat scratch disease in humans. Bartonella quintana is the etiologic agent of trench fever and has also been associated with bacteremia, endocarditis, and bacillary angiomatosis. The other Bartonella species infecting humans have mainly been implicated in endocarditis and neuroretinitis.

Description: BactoReal^® Kit Bartonella spp. is based on the amplification and detection of the gltA gene of B. clarridgeiae, B. elizabethae, B. grahamii, B. henselae, B. koehlerae, B. quintana, B. volans and B. washoensis using real-time PCR (detection in FAM channel, 530 nm).

PCR-platforms: BactoReal^® Kit Bartonella spp. is developed and validated for the ABI PRISM^® 7500 instrument (Life Technologies), LightCycler^® 480 (Roche) and Mx3005P^® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Sensitivity and specificity: See product description