Accutase Cell Detachment Solution, w: 0.5 mM EDTA, w: Phenol red

Accutase Cell Detachment Solution, w: 0.5 mM EDTA, w: Phenol red

Product description

Accutase ® is the gentle replacement for Trypsin in all adherent cell cultures and especially when working with sensitive cell types. Accutase ® is a non-mammalian, non-bacterial ready-to-use solution.

PAN-Biotech produces and distributes Accutase ® under license of the patentee Innovative Cell Technologies. Exclusive and for the whole of Europe.

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Composition

Accutase ® enzymes (activity > 500 U/ml) in DPBS w/o Ca/Mg with 0.5 mM EDTA and phenol red.

Suitability

Accutase ® can be used as a direct replacement of Trypsin for cell dissociation. Accutase ® is a ready-to-use cell detachment solution made of collagenolytic and proteolytic enzymes. It is used for routine detachment of adherent cells from tissue culture plates and flasks. A multitude of cell types has been successfully sub-cultured with Accutase ®, including fibroblasts, endothelial, vascular smooth muscle cells, as well as hepatocytes, embryonal stem cells, and many immortalized cell lines such as adherent CHO and BHK cells, HEK 293, L929, HeLa, 3T3, and others. Accutase ® is free of mammalian or bacterial products.

Gentle detachment of cells for:

• analysis of cell surface markers,
• transfection procedures,
• migration or proliferation assays,
• flow cytometry,
• and routine cell passage.

Special advantages

• Ready-to-use solution
• Gentle and effective cell detachment
• Lower contamination risk
• No neutralization required
• Protection of surface epitopes
• Maximum cell viability and enhanced plating efficiency
• Alternative to Trypsin