2-Nitrophenyl-B-D-galactopyranoside

2-Nitrophenyl-B-D-galactopyranoside

Product description

ONPG is a colorimetric and spectrophotometric substrate for detection of beta-galactosidase activity. ONPG is cleaved by beta-galactosidase via hydrolysis at the beta-1-4-glycosidic bond be-tween 2-nitrophenol and galactose. The released 2-nitro-p-henol is measured spectrophotometrically at 405 nm. The absorbance intensity at this wave-length is directly related to the specific activity.

Basic info

• CAS number 369-07-3
• Molecular weight C₁₂H₁₅NO₈ = 301.3
• Assay >90 %

Technical

Storage condition

• Store dry at 2-8 °C
• Soluble in water, DMSO and DMF
• Protect from light and moisture

Hazard statements

• H335 - May cause respiratory irritation

Precautionary statements

• P261 - Avoid breathing dust/fume/gas/mist/vapours/spray
• P304 - IF INHALED:
• P340 - Remove victim to fresh air and keep at rest in a position comfortable for breathing