BAC DNA MiniPrep Kit

Product code : NOR.18050

For the rapid preparation of BAC DNA

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Product description

For the rapid preparation of BAC DNA

Fast and easy processing using alcohol precipitation or a rapid spin-column format
High yield of plasmid DNA - up to 1 µg from 3 mL of culture
Versatile protocol that allows different volume of input to be processed

This kit is designed for the rapid preparation of BACs (bacterial artificial chromosomes) and other large DNA constructs from small batch cultures of Escherichia coli. Purification of constructs up to 130 kb in size have been verified. Rapid alcohol precipitation or spin-column purification allows for the processing of multiple samples in 1 hour. The DNA is preferentially purified from other cellular components such as genomic DNA and RNA. Typical DNA recoveries range between 0.6 µg and 1 µg from 3.0 mL of bacterial cultures. The purified DNA is fully digestible with all restriction enzymes tested, and is fully compatible with bacterial transformation and sequencing to achieve 95-100% accuracy.

Kit Specifications
Input Culture Volume	Alcohol Precipitation: Up to 100 mL Column Purification: Up to 5 mL
Column Binding Capacity	25 µg
Size of Plasmids Purified	Up to 130 kbp
Average Yield from 3 mL of Culture	0.6 - 1 µg
Time to Complete 10 Purifications	1 hour

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The RNAse should be stored at -20°C upon arrival. All the reagents should remain stable for at least 1 year in their unopened containers.

BAC DNA MiniPrep Kit

Product specifications

Packaging Unit

50 preps (1.5 mL)

Brand

Norgen biotek