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Blood DNA Isolation Kit (Magnetic Bead System)
Code produit: NOR.62600 NOR.62600
Norgen's Blood DNA Isolation Kit (Magnetic Bead System) is designed for the rapid preparation of genomic DNA from up to 200 µL of whole blood from various species, including human.
Packaging Unit
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Blood DNA Isolation Kit (Magnetic Bead System)
Product Description
- Isolate genomic DNA from cultured cells as well as various tissue types
- Fast, reproducible and easy processing using a magnetic bead system
- Isolate high quality genomic DNA
- Recovered genomic DNA is compatible with various downstream applications
- Also available in a 96-well format that can be integrated with a robotic automation system
Norgen's Blood DNA Isolation Kit (Magnetic Bead System) is designed for the rapid preparation of genomic DNA from up to 200 ’…L of whole blood from various species, including human. Purification is based on magnetic beads as the separation matrix. Norgen's magnetic beads bind DNA under optimized salt concentrations and releases the bound DNA under low salt and slightly alkali conditions. The genomic DNA is preferentially purified from other cellular proteinaceous components. Typical yields of genomic DNA will vary depending on the cell density of the blood sample. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with downstream applications including real-time PCR, NGS and microarray analysis.
Norgen's Blood DNA Isolation Kit (Magnetic Bead System) is also available in a 96-well format for high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.
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Kit Specifications
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Maximum Blood Input
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200 ’…L
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Average Yield (200
’…L blood)*
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4-12
’…g
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| Average Purity (OD260/280) |
1.8 - 1.9
|
| Time to Complete Purifications |
40 minutes (hands-on time Cat. 59800)
50 minutes (hands-on time Cat. 62600) |
* Average DNA yield will vary depending on the donor
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature (15 - 25’C). These reagents should remain stable for at least 2 years in their unopened containers.
| Component | Cat. 59800 (50 preps) |
|---|---|
| Lysis Buffer B | 20 mL |
| Proteinase K | 1.2 mL |
| Magnetic Bead Suspension | 2 x 1.1 mL |
| Solution WN | 18 mL |
| Elution Buffer B | 30 mL |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |
| Component | Cat. 62600 (2 x 96-well) |
| Lysis Buffer B | 1 x 40 mL 1 x 20 mL |
| Proteinase K in Storage Buffer | 4 mL |
| Magnetic Bead Suspension | 8.5 mL |
| Solution WN | 55 mL |
| Elution Buffer B | 1 x 30 mL 1 x 15 mL |
| 96-Well Plate | 2 |
| 96-Well Elution Plate | 2 |
| Adhesive Tape | 2 |
| Product Insert | 1 |
62600 - Protocol (2 x 96-well)
Norgen's Blood DNA Isolation Kit (Magnetic Bead System) is designed for the rapid preparation of genomic DNA from up to 200 ’…L of whole blood from various species, including human. Purification is based on magnetic beads as the separation matrix. Norgen's magnetic beads bind DNA under optimized salt concentrations and releases the bound DNA under low salt and slightly alkali conditions. The genomic DNA is preferentially purified from other cellular proteinaceous components. Typical yields of genomic DNA will vary depending on the cell density of the blood sample. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with downstream applications including real-time PCR, NGS and microarray analysis.
Norgen's Blood DNA Isolation Kit (Magnetic Bead System) is also available in a 96-well format for high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.
|
Kit Specifications
|
|
|
Maximum Blood Input
|
200 ’…L
|
|
Average Yield (200
’…L blood)*
|
4-12
’…g
|
| Average Purity (OD260/280) |
1.8 - 1.9
|
| Time to Complete Purifications |
40 minutes (hands-on time Cat. 59800)
50 minutes (hands-on time Cat. 62600) |
* Average DNA yield will vary depending on the donor
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature (15 - 25’C). These reagents should remain stable for at least 2 years in their unopened containers.
| Component | Cat. 59800 (50 preps) |
|---|---|
| Lysis Buffer B | 20 mL |
| Proteinase K | 1.2 mL |
| Magnetic Bead Suspension | 2 x 1.1 mL |
| Solution WN | 18 mL |
| Elution Buffer B | 30 mL |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |
| Component | Cat. 62600 (2 x 96-well) |
| Lysis Buffer B | 1 x 40 mL 1 x 20 mL |
| Proteinase K in Storage Buffer | 4 mL |
| Magnetic Bead Suspension | 8.5 mL |
| Solution WN | 55 mL |
| Elution Buffer B | 1 x 30 mL 1 x 15 mL |
| 96-Well Plate | 2 |
| 96-Well Elution Plate | 2 |
| Adhesive Tape | 2 |
| Product Insert | 1 |
Blood DNA Isolation Kit (Magnetic Bead System)
Spécifications du produit
Packaging Unit
2 x 96-well plates
Brand
Norgen biotek
Category
Blood/Plasma/Serum Sample Preparation Kits
Purification
DNA Purification