BactoReal® Kit Brachyspira hyodysenteriae

BactoReal® Kit Brachyspira hyodysenteriae

Product Description

Kit contents:

• Detection assay for B. hyodysenteriae
• Detection assay for internal positive control (control of amplification)
• DNA reaction mix (contains uracil-N glycosylase, UNG)
• Positive control for B. hyodysenteriae
• Water

Background: Brachyspira hyodysenteriae (previously called Serpulina hyodysenteriae or Treponema hyodysenteriae) is an anaerobic intestinal spirochaete and the causative agent of swine dysentery. Swine dysentery is a disease characterized by mucohaemorrhagic diarrhoea with lesions confined to the large intestine of pigs. Its incubation time is 10-14 days. The faecal excretion of B. hyodysenteriae starts 2 days after infection. Brachyspira hyodysenteriae is an anaerobic bacterium but is aerotolerant due, at least in part, to high NADH oxidase activity.

Description: BactoReal^® Kit Brachyspira hyodysenteriae is based on the amplification and detection of the nox gene of B. hyodysenteriae using real-time PCR.

PCR-platforms: BactoReal^® Kit Brachyspira hyodysenteriae is developed and validated for the ABI PRISM^® 7500 instrument (Life Technologies), LightCycler^® 480 (Roche) and Mx3005P^® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Sensitivity and specificity: See product description