BactoReal® Kit Bordetella bronchiseptica

BactoReal® Kit Bordetella bronchiseptica

Product Description

Kit contents:

• Detection assay for Bordetella bronchiseptica
• Detection assay for internal positive control (control of amplification)
• DNA reaction mix (contains uracil-N glycosylase, UNG)
• Positive control for B. bronchiseptica
• Water

Background: Bordetella bronchiseptica is a gram-negative bacterium found as a normal inhabitant of the respiratory tract in a variety of animals such as dogs, pigs, some rodents, as well as humans. In some cases it causes pneumonia, canine infectious tracheobronchitis (kennel cough), bronchopneumonia in cats, and atrophic rhinitis in pigs. It is widely distributed in the swine population. Here, some strains cause a mild and non progressive rhinitis that heals spontaneously. However if toxigenic pasteurella are present in the herd then a combination of the two organisms can produce severe progressive rhinitis (PAR). Bordetella bronchiseptica can also be a secondary opportunist invader in pneumonia.

Description: BactoReal^® Kit Bordetella bronchiseptica is based on the amplification and detection of the bfrA gene of B. bronchiseptica using real-time PCR.

PCR-platforms: BactoReal^® Kit Bordetella bronchiseptica is developed and validated for the ABI PRISM^® 7500 instrument (Life Technologies), LightCycler^® 480 (Roche) and Mx3005P^® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Sensitivity and specificity: See product description