ViroReal® Kit Bovine Nebovirus

ViroReal® Kit Bovine Nebovirus

Product Description

Kit contents:

• Detection assay for bovine nebovirus
• Detection assay+target for internal RNA positive control (control of RT-PCR/RNA extraction)
• RNA reaction mix
• Nuclease-free water
• Positive control (RNA) for bovine nebovirus

Background: Neboviruses are non-enveloped, single-stranded, linear, positive-sense RNA viruses ((+)ssRNA), belonging to the Caliciviridae family. The only currently known species in the genus Nebovirus is Newbury-1 virus. Cattle, especially calves, serve as natural hosts. Neboviral infection is associated with gastrointestinal disorders and necrotizing hepatitis leading to fatal hemorrhages in calves. The disease often remains endemic due to the lack of a commercially available vaccine.

Description: ViroReal^® Kit Bovine Nebovirus is based on the amplification and detection of the ORF 1 of bovine nebovirus using one-step reverse transcription real-time PCR.

PCR-platforms: ViroReal^® Kit Bovine Nebovirus is developed and validated for the ABI PRISM^® 7500 instrument (Life Technologies), LightCycler^® 480 (Roche) and Mx3005P^® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Specificity and sensitivity: See product description