ParoReal® Kit Neospora caninum

ParoReal® Kit Neospora caninum

Product Description

Kit contents:

• Detection assay for Neospora caninum
• Detection assay for internal positive control (control of amplification)
• DNA reaction mix (contains uracil-N glycosylase, UNG)
• Positive control for Neospora caninum
• Water

Background: Neospora caninum is a coccidian parasite and an important cause of spontaneous abortion in infected livestock. It has a heteroxenous life cycle, with the sexually reproductive stage occurring in the intestine of a definitive host (domestic dog, coyotes, gray wolves and dingos). Oocysts passed in the feces of the definitive host are ingested by intermediate hosts, such as cattle. Formation of cysts results in chronic infection of the intermediate host. Neosporosis is an infectious disease for many different canids and cattle. In canines it leads to neuromuscular degeneration. In dairy and beef cattle it is an important cause of reproductive problems and abortion. Once the cattle is infected, N. caninum is maintained as a life-long infection and is transmissed from cow to the fetus during pregnancy, resulting in abortion or weak or also normal calves. The occurrence of N. caninum infection in beef and dairy cattle has been reported worldwide.

Description: ParoReal Kit Neospora caninum is based on the amplification and detection of the NC5 gene of Neospora caninum using real-time PCR.

PCR-platforms: ParoReal Kit Neospora caninum is developed and validated for the ABI PRISM^® 7500 instrument (Life Technologies), LightCycler^® 480 (Roche) and Mx3005P^® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Sensitivity and specificity: See product description