ParoReal® Kit Cryptosporidium parvum

ParoReal® Kit Cryptosporidium parvum

Product Description

Kit contents:

• Detection assay for Cryptosporidium parvum
• Detection assay for internal positive control (control of amplification)
• DNA reaction mix (contains uracil-N glycosylase, UNG)
• Positive control for Cryptosporidium parvum
• Water

Background: Cryptosporidium parvum is one of several protozoal species that cause cryptosporidiosis, a parasitic disease of the mammalian intestinal tract. It is a zoonotic and obligate intracellular parasite. Primary symptoms of C. parvum infection are acute, watery, and non-bloody diarrhea. C. parvum infection is of particular concern in immunocompromised patients. Extra-intestinal sites include the lung, liver and gall bladder where it causes respiratory cryptosporidosis, hepatitis and cholecystitis. Infection is caused by ingestion of sporulated oocysts transmitted by the faecal-oral route.

Description: ParoReal Kit Cryptosporidium parvum is based on the amplification and detection of the GP60 gene of Cryptosporidium parvum using real-time PCR.

PCR-platforms: ParoReal Kit Cryptosporidium parvum is developed and validated for the ABI PRISM^® 7500 instrument (Life Technologies), LightCycler^® 480 (Roche) and Mx3005P^® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Sensitivity and specificity: See product description