CHÉE & POOL medium including vitamins

CHÉE & POOL medium including vitamins

Product Description

In the medium defined by Chée and Pool the original Murashige and Skoog concentration of Chlorine, Iodine and Manganese is decreased, resulting in an improved shoot multiplication of Vitis. Substituting calcium nitrate for calcium chloride improved the quality of grapevine shoots produced in culture. Shoot multiplication was dramatically improved by ommitting Iodine and lowering the concen-tration of Manganese. This might be the result of the involvement of both ions in auxin metabolism and tranport.

Composition

Micro Elements	mg/l	µM
CoCl2.6H2O	0.025	0.11
CuSO4.5H2O	0.025	0.10
FeNaEDTA	36.70	100.00
H3BO3	6.20	100.27
MnSO4.H20	0.85	5.00
Na2MoO4.2H2O	0.25	1.03
ZnSO4.7H2O	8.60	29.91

Macro Elements	mg/l	mM
Ca(NO3)2	492.30	2.99
KH2PO4	170.00	1.25
KNO3	1900.00	18.79
MgSO4	180.54	1.50
NH4NO3	1650.00	20.61

Vitamins	mg/l	µM
myo-Inositol	10.00	55.5
Nicotinic Acid	1.00	8.12
Pyridoxine HCl	1.00	5.00
Thiamine HCl	1.00	3.00

Total concentration Micro and Macro elements including -vitamins: 4458.49 mg/l

Chée, R., and Pool, R.M., Improved Inorganic Media Constituents for In Vitro Shoot Multiplication of Vitis, Scientia Horticulturae, 32 (1987) 85-95.