Anderson's Rhododendron including vitamins

Anderson's Rhododendron including vitamins

Product Description

A two-fold increase in shoot multiplication of red raspberry on Anderson's medium was achieved as compared to the Murashige and Skoog formulation. A comparison of the inorganics of both formulations showed a reduction to approximately 1/4 strength on NH4NO3 and KNO3 in Anderson inorganics. The optimal concentrations of growth- regulators for shoot multiplication of red and black raspber-ry were 0.1-2.5 µM IBA and 4.5-9.0 µM BA. In vitro rooting of black and red raspberries were succes-ful using the basal media that included Anderson's Inorganics, 5µM IBA and 600 mg/l activated charcoal. The latter is essential for high rooting percentagesA two-fold increase in shoot multiplication of red raspberry on Anderson's medium was achieved as compared to the Murashige and Skoog formulation. A comparison of the inorganics of both formulations showed a reduction to approximately 1/4 strength on NH4NO3 and KNO3 in Anderson inorganics. The optimal concentrations of growth- regulators for shoot multiplication of red and black raspber-ry were 0.1-2.5 µM IBA and 4.5-9.0 µM BA. In vitro rooting of black and red raspberries were succes-ful using the basal media that included Anderson's Inorganics, 5µM IBA and 600 mg/l activated charcoal. The latter is essential for high rooting percentagesA two-fold increase in shoot multiplication of red raspberry on Anderson's medium was achieved as compared to the Murashige and Skoog formulation. A comparison of the inorganics of both formulations showed a reduction to approximately 1/4 strength on NH4NO3 and KNO3 in Anderson inorganics. The optimal concentrations of growth- regulators for shoot multiplication of red and black raspber-ry were 0.1-2.5 µM IBA and 4.5-9.0 µM BA. In vitro rooting of black and red raspberries were succes-ful using the basal media that included Anderson's Inorganics, 5µM IBA and 600 mg/l activated charcoal. The latter is essential for high rooting percentages

Composition

Micro Elements	mg/l	µM
CoCl2.6H2O	0.025	0.11
CuSO4.5H2O	0.025	0.10
FeNaEDTA	73.40	200.00
H3BO3	6.20	100.27
KI	0.30	1.81
MnSO4.H2O	16.90	100.00
Na2MoO4.2H2O	0.25	1.03
ZnSO4.7H2O	8.60	29.91

Macro Elements	mg/l	mM
CaCl2	332.02	2.99
KNO3	480.00	4.75
MgSO4	180.54	1.50
NaH2PO4	330.60	2.75
NH4NO3	400.00	5.00

Vitamins	mg/l	µM
Adenine sulphate	80.00	197.87
myo-Inositol	100.00	554.94
Thiamine HCl	0.40	1.19

Total concentration Micro and Macro elements including -vitamins: 2009.26 mg/l

Anderson W.C., Tissue culture propagation of Red and Black Raspberries, Rubus Idaeus and R. Occidentalus Act. Hort., 112, 13 (1980).