Anderson's Rhododendron

Anderson's Rhododendron

Product Description

A two-fold increase in shoot multiplication of red raspberry on Anderson's medium was achieved as compared to the Murashige and Skoog formulation. A comparison of the inorganics of both formulations showed a reduction to approximately 1/4 strength on NH4NO3 and KNO3 in Anderson inorganics. The optimal concentrations of growth- regulators for shoot multiplication of red and black raspber-ry were 0.1-2.5 µM IBA and 4.5-9.0 µM BA. In vitro rooting of black and red raspberries were succes-ful using the basal media that included Anderson's Inorganics, 5µM IBA and 600 mg/l activated charcoal. The latter is essential for high rooting percentagesA two-fold increase in shoot multiplication of red raspberry on Anderson's medium was achieved as compared to the Murashige and Skoog formulation. A comparison of the inorganics of both formulations showed a reduction to approximately 1/4 strength on NH4NO3 and KNO3 in Anderson inorganics. The optimal concentrations of growth- regulators for shoot multiplication of red and black raspber-ry were 0.1-2.5 µM IBA and 4.5-9.0 µM BA. In vitro rooting of black and red raspberries were succes-ful using the basal media that included Anderson's Inorganics, 5µM IBA and 600 mg/l activated charcoal. The latter is essential for high rooting percentagesA two-fold increase in shoot multiplication of red raspberry on Anderson's medium was achieved as compared to the Murashige and Skoog formulation. A comparison of the inorganics of both formulations showed a reduction to approximately 1/4 strength on NH4NO3 and KNO3 in Anderson inorganics. The optimal concentrations of growth- regulators for shoot multiplication of red and black raspber-ry were 0.1-2.5 µM IBA and 4.5-9.0 µM BA. In vitro rooting of black and red raspberries were succes-ful using the basal media that included Anderson's Inorganics, 5µM IBA and 600 mg/l activated charcoal. The latter is essential for high rooting percentages

Composition

Micro Elements	mg/l	µM
CoCl2.6H2O	0.025	0.11
CuSO4.5H2O	0.025	0.10
FeNaEDTA	73.40	200.00
H3BO3	6.20	100.27
KI	0.30	1.81
MnSO4.H2O	16.90	100.00
Na2MoO4.2H2O	0.25	1.03
ZnSO4.7H2O	8.60	29.91

Macro Elements	mg/l	mM
CaCl2	332.02	2.99
KNO3	480.00	4.75
MgSO4	180.54	1.50
NaH2PO4	330.60	2.75
NH4NO3	400.00	5.00

Anderson W.C., Tissue culture propagation of Red and Black Raspberries, Rubus Idaeus and R. Occidentalus Act. Hort., 112, 13 (1980).