Mouse on Mouse (M.O.M.TM) Fluorescein Kit

Mouse on Mouse (M.O.M.TM) Fluorescein Kit

Product Description

The Vector^® M.O.M.™ Immunodetection Kits are specifically designed to reduce endogenous mouse IgG staining when using mouse primary antibodies on mouse tissue.  The inability of an anti-mouse secondary antibody to distinguish between a primary antibody produced in mice and the endogenous mouse immunoglobulins present in mouse tissue results in high background staining which obscures specific staining.  This problem can be essentially eliminated by using a Vector^® M.O.M.™ Kit.  The result is clear, crisp specific staining of the antigens of interest.

The Vector^® M.O.M.™ Fluorescein Kit contains a proprietary Mouse IgG Blocking Reagent, a M.O.M.™ Biotinylated Anti-Mouse Ig Reagent, and Fluorescein Avidin DCS and is based on a simple and easy to follow avidin/biotin protocol; no tedious calculations or antibody pre-binding steps are required.  Excellent staining results for a once difficult application have now become routine with the Vector^® M.O.M.™ System.

Key advantages of the Vector^® M.O.M.™ Immunodetection Kits:

• Significant reduction of endogenous mouse Ig staining when using mouse primary antibodies on mouse tissue
• Clear, crisp specific staining of antigens of interest
• Based on VECTASTAIN^® ABC reagent protocols
• Procedures are simple and easy to follow
• No tedious calculations or antibody prebinding steps required

The Vector^® M.O.M.™Immunodetection Kits are useful in any mouse-on-mouse application, such as studies in genetically engineered mice, including transgenic and knock-out models, as well as mouse xenografts.

The M.O.M. Kits can be also used in multiple antigen staining protocols to localize two different mouse primary antibodies on the same mouse tissue sections using fluorescent or enzyme-based detection systems. Detailed information for multiple antigen labeling can be found in our brochure, Discovery Through Color: A Guide to Multiple Antigen Labeling , available on our website.

Four Vector^® M.O.M.™ Kits are available. The three kits based on avidin/biotin technology use the same proprietary M.O.M.™ Mouse Ig Blocking Reagent and the M.O.M.™ Biotinylated Anti-Mouse Ig Reagent, but offer a choice of an enzyme-based or fluorescent-based visualization method. M.O.M.™ Mouse Ig Blocking Reagent and M.O.M.™ Biotinylated Anti-Mouse Ig Reagent are also available separately.

The Vector^® M.O.M.™ ImmPRESS™ Kit contains our proprietary M.O.M.™ Mouse Ig Blocking Reagent paired with a specialized, ready-to-use, one-step, non-biotin M.O.M.™ ImmPRESS™ Peroxidase Polymer reagent.

Vector^® M.O.M.™ Fluorescein Kit (FMK-2201) includes the following:

• M.O.M.™ Mouse Ig Blocking Reagent
• M.O.M.™ Biotinylated Anti-Mouse Ig Reagent
• M.O.M.™ Protein Concentrate for general protein blocking and diluent
• Fluorescein Avidin DCS (green fluorescence, excitation at 495 nm; emission at 515 nm)

This Vector^® M.O.M.™ kit contains sufficient stock reagents to provide approximately 25 ml of working solution which is usually enough to stain about 125-250 mouse tissue sections.