ImmPRESS Duet Double Staining HRP/AP Polymer Kit (anti-rabbit IgG-brown, anti-mouse IgG-red)

ImmPRESS Duet Double Staining HRP/AP Polymer Kit (anti-rabbit IgG-brown, anti-mouse IgG-red)

Product Description

Save time and streamline your double staining IHC workflow with the ImmPRESS™ Duet Double Staining Polymer Kit. This kit enables fast, well-defined localization and visualization of two different target antigens on the same tissue section. Utilizing our unique ImmPRESS™ micropolymer chemistry, we independently conjugate highly-active horseradish peroxidase (HRP) and alkaline phosphatase (AP) enzyme polymers to anti-mouse IgG and anti-rabbit IgG affinity-purified, highly cross-adsorbed secondary antibodies. These reagents are then blended at optimized dilutions to provide a stable pre-diluted, ready-to-use HRP/AP formula that will detect mouse and rabbit primary antibodies, and facilitate a time-saving, simplified, one-step double label detection protocol. For maximum sensitivity and staining contrast between target antigens, ImmPACT™ DAB EqV (HRP, brown) and ImmPACT™ Vector® Red (AP, magenta) substrates are included to complete this easy to use staining kit.

Key advantages

• Complete double staining HRP/AP Kits
• Highly cross-adsorbed, non-biotin polymer detection reagent
• High sensitivity and specificity
• Prediluted, ready- to-use in convenient dropper bottles

Kit includes

1. 15 ml BLOXALL™ Blocking Solution
2. 15 ml 2.5% Normal Serum
3. 15 ml ImmPRESS™ Duet HRP/AP Polymer Reagent (mixture of ImmPRESS™ HRP Anti-Rabbit IgG and ImmPRESS™ AP Anti-Mouse IgG)
4. ImmPACT™ DAB EqV Substrate (HRP, brown)
5. ImmPACT™ Vector® Red Substrate (AP, magenta)

The ImmPRESS™ Duet Double Staining Polymer Kit is intended for use on non-rodent tissue specimens. Reliable and reproducible results are obtained on tissue sections where the two target antigens do not over-lap (co-localize) in the same structure of the same cell, but rather are expressed in different cell compartments or different cell types.

Also included, BLOXALL™ Blocking Solution abolishes all endogenous peroxidase and alkaline phosphatase activity in a single 10 minute incubation. In addition, 2.5% Normal Serum blocks all non-specific binding of detection reagents to ensure crisp, specific staining.

All reagents are supplied in stabilized, ready-to-use (R.T.U.) solutions for maximum convenience, and consistency between assays. The ImmPRESS Duet Double Staining Polymer Kits offer a complete and simple solution for visualizing target antigens in cell and tissue preparations and contains sufficient reagents to stain approximately 150 sections.

 

Learn more about the ImmPRESS™ Polymer Detection Systems in double labeling.

Product review on Biocompare.com.