Cells and Tissue DNA Isolation Kit

Code produit : NOR.53100

For the rapid preparation of genomic DNA from cultured cells as well as various tissue samples.

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Cells and Tissue DNA Isolation Kit

Product Description

For the rapid preparation of genomic DNA from cultured cells as well as various tissue samples.

Rapid spin column format
High yield
Excellent quality
DNA ready for any application including PCR, qPCR, genotyping and more

This kit is designed for the purification of genomic DNA from cultured cells, viruses and various tissue samples using rapid and convenient spin columns. Purified DNA is of an excellent yield and quality and is immediately ready for any downstream application including PCR, qPCR, genotyping and more. The protocol can be completed in approximately 30 minutes for cells and within 90 minutes for tissues.

Kit Specifications
Maximum Input	20 mg of animal tissue Up to 150µL of viral suspension 3 x 10⁶ cells
Column Binding Capacity	> 50µg
Average Yield	8µg (from 1 x 10⁶ HeLa Cells) 10µg (from 10 mg kidney)
Elution Volume	50 - 200µL
Analyte Purified	Genomic DNA, mitochondrial DNA, viral DNA
Format	Spin Column
Time to Complete 10 Purifications	30 min (cells) and 90 min (tissue)

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 2 years in their unopened containers. The kit contains a ready-to-use Proteinase K solution, which is dissolved in a specially prepared storage buffer. The Proteinase K is stable for up to 1 year after delivery when stored at room temperature. To prolong the lifetime of Proteinase K, storage at 2-8°C is recommended.

Component	Cat. 53100 (50 preps)
Lysis Buffer B	20 mL
Solution WN	18 mL
Wash Solution A	18 mL
Elution Buffer B	30 mL
Proteinase K	1.2 mL
Spin Columns	50
Collection Tubes	50
Elution Tubes (1.7 mL)	50
Product Insert	1

Cells and Tissue DNA Isolation Kit

Spécifications du produit

Packaging Unit

50 preps

Brand

Norgen biotek