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Biofilm DNA Isolation Kit
Code produit : NOR.62300
NOR.62300Rapid and convenient method to isolate genomic DNA from different types of biofilm and biofilm forming-bacteria
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Biofilm DNA Isolation Kit
Product Description
- Rapid and convenient method to isolate genomic DNA from different types of biofilm and biofilm forming-bacteria
- No phenol or chloroform extractions
- Yields high quality DNA that is ready for PCR and other downstream applications
Norgen's Biofilm DNA Isolation Kit is designed for the rapid preparation of genomic DNA from biofilm. Genomic DNA is efficiently extracted from the biofilm by a combination of heat treatment, detergents and the use of provided Bead Tubes. The kit is able to remove known PCR inhibitors typically found within the biofilm, including humic acid. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with downstream applications such as PCR and Southern Blot analysis. Preparation time for a single sample is less than 45 minutes, and each kit contains sufficient materials for 50 preparations.
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Kit Specifications
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| Maximum Column Binding Capacity |
50 µg
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| Maximum Column Loading Volume |
650 µL
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| Minimum Biofilm Input (wet weight) | 50 mg |
| Maximum Biofilm Input (wet weight) |
200 mg
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| Average Yield from 200 mg of biofilm* |
2-4 µg
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| Time to Complete 10 Purifications | 45 minutes |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 2 years in their unopened containers.
| Component | Cat. 62300 (50 preps) |
|---|---|
| Lysis Buffer L | 30 mL |
| Lysis Additive A | 6 mL |
| Binding Buffer I | 7 mL |
| Wash Solution A | 18 mL |
| Elution Buffer B | 8 mL |
| Bead Tubes | 50 |
| Spin Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |
Biofilm DNA Isolation Kit
Spécifications du produit
Packaging Unit
50 preps
Brand
Norgen biotek
Category
Genomic DNA Purification Kits
Purification
DNA Purification