APT Agar (All Purpose Tween)

APT Agar (All Purpose Tween)

Product Description

For the enumeration and cultivation of heterofermentative lactic acid bacteria including Lactobacilli, Streptococci Leuconostocs and lactic acid as well as microorganisms with high requirements of thiamine in meat products

Applications

• Cultivation / Enumeration
• LACTIC ACID BACTERIA

Packaging

Suspend 59,5 grams of the medium in one litre of destilled water. Add 0.1 grams of actidione (dissolved in a minimum amount of 40% ethanol) and 0.2 ml of Tween 80. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121°Cfor 15 minutes.

Formula

• Peptone 12.5
• Yeast Extract 7.5
• D(+)Glucose 10
• Sodium Citrate 5
• Thiamine Hydrochloride 0.001
• Sodium Chloride 5
• Dipotassium Hydrogen phosphate 5
• Manganese Chloride 0.8
• Ferrous sulfate 0.04
• Magnesium sulfate 0.14
• Agar 13.5