ViroReal® Kit Bovine Torovirus 

ViroReal® Kit Bovine Torovirus 

Product Description

Kit contents:

• Detection assay for bovine torovirus
• Detection assay+target for internal RNA positive control (control of RT-PCR/RNA extraction)
• RNA reaction mix
• Nuclease-free water
• Positive control (RNA) for bovine torovirus

Background: The species bovine torovirus (BToV) is a member of the family Coronaviridae, subfamily Torovirinae. The genome is a single-stranded, positive-sense RNA molecule. The kidney-shaped BToV causes diarrhea in calves but respiratory infections have also been reported and it has a worldwide distribution. Bovine torovirus, formerly called breda virus (BRV), was originally isolated from diarrheic calves in Breda, Iowa, in 1979.

Description: ViroReal^® Kit Bovine Torovirus is based on the amplification and detection of the membrane protein (M) gene of the BToV using one-step reverse transcription real-time PCR.

PCR-platforms: ViroReal^® Kit Bovine Torovirus is developed and validated for the ABI PRISM^® 7500 instrument (Life Technologies), LightCycler^® 480 (Roche) and Mx3005P^® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Specificity and sensitivity: See product description