StainIN™ eco-RED Nucleic Acid Stain

StainIN™ eco-RED Nucleic Acid Stain, a 10000X concentrated aqueous solution is a significantly safer alternative to ethidium bromide. It is same easy to use, twice as sensitive and much more secure. At least twice as economical as competing products, this novel DMSO-free stain can be also used and disposed with less environmental and health concerns compared to ethidium bromide. StainIN™ eco-RED is a fluorescent dye that allows detection of >0,1 ng of DNA in both agarose and polyacrylamide gels. It binds to both ds DNA, ssDNA and RNA and emits red fluorescence detectable under the LED or UV light and documented with same filters as ethidium bromide. For cloning applications, LED light is recommended. The lower than ethidium bromide carcinogenicity of the dye has been proven by Ames-test. 

The carcinogenicity and a high toxicity of a widely used gel stain Ethidium Bromide (EtBr) is one of the biggest safety and environmental problems in the labs where gel electrophoresis is a daily routine. Therefore, there is a need for a safer ethidium bromide alternatives to be used in laboratories. The novel less toxic nucleic acid dyes used for agarose gel electrophoresis address this ethidium bromide problem and minimize environmental concerns.

highQu StainIN™ eco-RED Nucleic Acid Stain provides a significantly safer alternative to ethidium bromide while delivering up to several times higher detection sensitivity. Same like ethidium bromide, the stain is used during the process of electrophoresis in an agarose gel or PAAG and (optionally) in the electrophoresis running buffer, or alternatively, for a post-run staining of the gels in a tray with the buffer. 

Despite having a proven lower mutagenicity, all nucleic acid stains shall be handled with care, as they interact with DNA and RNA.  Used electrophoresis buffer with a stain as well as melted gels shall be run through approved (carbon) filters. If the absence of residual fluorescence is confirmed, the liquids can be disposed with plenty of water down the drain. 

Alternatively, all used liquids can be autoclaved or heated as the StainIN™ eco-RED Nucleic Acid Stain degrades during the long exposure to above 100ºC temperature. 

The safety office shall be consulted periodically to match local regulations, as these regulations vary and change. Despite of tests described in this report, the seller of the listed stains and other reagents does not take any responsibility for the possible damage resulting from handling any sold chemical or reagent.